ISS Alba Frequency Domain Lifetime Imaging system [Funded by NIH RR 027409; 2009]

Alba FCS is a dual-channel spectrometer that combines a confocal scanning microscope with fluorescence correlation spectroscopy (FCS), and is optimized for both single-photon and multi-photon excitation. For digital frequency domain FLIM, the system has ISS Alba V 2 channel filter based detection and allows phasor plot analysis of fluorescence lifetime images. It gives the ability for analytical assaying of nano- to pico-molar concentrations in small volumes (~1 µm3). Other applications include quantitative analysis of diffusions and molecular kinetics in live specimens.

Components & Features

• Multi-laser line dichroics for FCCS or dual-color FCS
• FCS acquisition at multiple positions in time series
• Objective lenses: 20x, 60x (Water), 100x (Oil)
• Excitation lasers: 375nm, WLL(440-700nm, filter based)