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Olympus Motorized wide-field epi-fluorescence microcopy system

Olympus microscope
widefield diagram

Motorized wide-field epi-fluorescence microcopy system [Funded by UVA; 1996]

Olympus (IX-70) Infinity corrected inverted microscope with four output ports equipped with

High Content Screening system (HCS)

HCS system is based on the Olympus wide-field microscopy system. Max 96 well plate can be used to screen specimens. Metamorph software is used to drive the hardware and data acquisition.

Components & Features

  • Motorized x-, y-, and z-axis stage and filter wheels (Ex/Em/ND) from Prior
  • Many filter cubes are available covering the visible spectrum
  • Orca-2 (1280x1024, 12/14bit, 10MHz or 1.25MHz readout, ROI selectable) dual scan cooled digital Interline Hamamatsu CCD Camera
  • Microinjection device: Eppendorf
  • Temperature controlled and perfusion chambers to maintain the viability of the cells/tissue (Bioptech, 1 unit; Medical Systems Corp.)
  • Metamorph software.
  • Objective lenses: 2X, 4X/0.13, 10X/0.40, 20X/0.40, 40X/0.60 Oil, 60X/1.20 WI (PSF), 60X/1.40 Oil, 100X/1.40 Oil, Phase contrast Optics