Proceedings by Year

2022

  1. Alam, S.R., Wallrabe, H., Christopher, K.G., Siller, K., and Periasamy, A. (2022) Characterization of phototoxic effects in multiphoton FLIM. Proc. SPIE 11965, Multiphoton Microscopy in the Biomedical Sciences XXII, Proc. SPIE Vol. 11965.

2021

  1. Zhang, J., Wallrabe, H., Siller, K., Alam, S.R., Weller, D. and Periasamy, A. (2021) Machine learning architecture to predict drug response based on cancer cell FLIM images. Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 116481G (5 March 2021); doi:10.1117/12.2584042

2019

  1. Cao, R., Wallrabe, H., Huang, C., Tan, Su-Fern, Feith, D., Xing, Y., Wang, Y., Oberholzer, J., Kester, M., Loughran, T.P. and Periasamy, A. FLIM Imaging of NAD(P)H to track metabolic changes of non-adherent leukemia cells using micro cell trapping arrays. Proc. SPIE. Vol. 10882, 2019.

2018-Prior

  1. Chandler, L., Chandler, A. and Periasamy, A. (2018) Novel Snapshot Hyperspectral Imager for Fluorescence Imaging. Proc. SPIE. Vol. 10498.

  2. Periasamy, A., Alam, S.R., Svidrych, Z. and Wallrabe, H. (2017) FLIM-FRET Image Analysis of Tryptophan in Prostate Cancer Cells, Proc SPIE (ECBO) 10414: 1041402-Pp1-5.

  3. Alam, S.R., Wallrabe, H., Svindrych, Z., Chandra, D. and Periasamy, A. (2017) Effects of Anti-Cancer Drug Doxorubicin on Endogenous Biomarkers NAD(P)H, FAD & Trp in prostate cancer cells- a FLIM Study. Proc. of SPIE, Vol. 10069: 100691L-Pp1-6.  

  4. Chandler, A., Chandler, A., Wallrabe, A. and Periasamy, A. (2017) Differential levels of metabolic activity in isolated versus confluent/partially confluent HeLa cells are analyzed by autofluorescent NAD(P)H using multi-photon FLIM microscopy. Proc. SPIE Int. Soc. Opt. Eng. 10069: 100692R-Pp1-6.

  5. Rehman, S., O’Melia, M.J., Wallrabe, H., Svindrych, Z. and Periasamy, A. (2016) Investigation of prostate cancer cells using NADH and Tryptophan as biomarker: multiphoton FLIM-FRET microscopy. Proc. SPIE Int. Soc. Opt. Eng.7712: 97120Q. pp1-5.

  6. O’Melia, M.J., Wallrabe, H., Svindrych, Z., Rehman, S. and Periasamy, A. (2016) FLIM data analysis of NADH and tryptophan autofluorescence in prostate cancer cells. Proc. SPIE Int. Soc. Opt. Eng.9712: 97122E. pp 1-6.

  7. Rehman, S., Brown-Steinke, K., Palmer, L., Periasamy, A. (2015) Confocal immunofluorescence FRET microscopy to investigate eNOS and GSNOR localization and interaction in pulmonary endothelial cells. Proc. SPIE Int. Soc. Opt. Eng. 9329: 93290G.

  8. Wallrabe, H., Svindrych, Z. and Periasamy, A. (2015) Comprehensive quantitative evaluation of FLIM-FRET image data using intensity-based analysis tools. Proc. SPIE Int. Soc. Opt. Eng. 9329: 93293A. pp1-8.

  9. Sun, Y. and Periasamy, A. (2012) Monitoring Protein-Protein Interactions in Living Specimens using FLIM-FRET Microscopy. Proc. Microsc. Microanal. 18 (Suppl2):148-149.

  10. Sun, Y., Wang, L., Jyothikumar, V., Brautigan, D. B. and Periasamy, A. (2012) Characterizing the Interactions between Prolyl Isomerase Pin1 and Phosphatase Inhibitor-2 in Living Cells with FRET and FCS. Proc. SPIE Int. Soc. Opt. Eng. 8226: pp.82263T.

  11. Horst, W., Sun, Y., Fang, X., Periasamy, A. and Bloom, G. (2012) Three-color FRET expands the ability to quantiy the interactions of several proteins involve in actin filament nucleation. Proc SPIE Int Soc Opt Eng.  8226: pp.82260J.

  12. Sun, Y. Seo, S., Provence, S. and Periasamy, A. (2010) Comparison of FRET microscopy imaging techniques for studying protein-protein interactions in living cells using FRET standards. Proc. SPIE Int. Soc. Opt. Eng. 7569: 7569

  13. Sun, Y., Booker, C.F., Day, R.N. and Periasamy, A. (2009) Localization of protein-protein interactions among three fluorescent proteins in a single living cell: three-color FRET microscopy. Proc. SPIE Int. Soc. Opt. Eng.  7183: 71832E. 

  14. Wallrabe, H., Periasamy, A. and Barroso, M. (2009) FRET imaging of multiple focal planes to analyze the organization and conformation of tranferrin-receptor in polarized cells. Proc. SPIE Int. Soc. Opt. Eng. 7183: 71832F-(1-6).

  15. Day, R. N., Sun, Y., Booker, C. Y., Kumari, S., Periasamy, A. and Davidson, M. (2009) The characterization of optimized fluorescent proteins for Forster resonance energy transfer microscopy. Proc. SPIE Int. Soc. Opt. Eng. 7183: 718306.

  16. Li, H., Yu, P., Felder, R.A., Periasamy, A. and Jose, P.A. (2009) Angiotension II-induced angiotension II type I receptor lysosomal degradation studied by fluorescence lifetime imaging microscopy. Proc. SPIE Int. Soc. Opt. Eng. 7183: 71832G. 

  17. Chen, Y. and Periasamy, A. (2007) Acceptor spectral bleedthrough correction in spectral FRET imaging microscopy. Proc. SPIE Int. Soc. Opt. Eng. 6442. 644223.

  18. Yen, W., Burdsal, C., Periasamy, A. and Sutherland, A.E. (2006) Axial elongation in mouse embryos involves mediolateral cell intercalation behavior in the paraxial mesoderm. Proc. SPIE Int. Soc. Opt. Eng. 6089:60891K.

  19. Wallrabe, H., Periasamy, A. and Barroso, M. (2006) 2-P FLIM-FRET in combination with confocal FRET provides additional insights of the organization of Transferrin receptor-ligand complexes in endocytic membranes of polarized MDCK cells. Proc. SPIE Int. Soc. Opt. Eng. 6089:608905.

  20. Chen, Y. and Periasamy, A. (2006) Quantitative FRET data analysis: Protein localization in living specimens. Proc. SPIE Int. Soc. Opt. Eng. 6089:60891W.

  21. Chen, Y., King, M., and Periasamy, A. (2005) One- and two-component analysis of cyan fluorescent protein: FLIM-FRET microscopy. Proc. SPIE Int. Soc. Opt. Eng. 5700:279-285.

  22. Chen, Y and Periasamy, A. (2004) Two-photon lifetime imaging microscopy for protein localization. Proc. SPIE Int. Soc. Opt. Eng. 5323, 431-439.

  23. Chen, Y., Majid, S., Day, R.N. and A. Periasamy (2003) Two-photon excitation lifetime imaging and excitation spectra of green fluorescent proteins. Proc. SPIE Int. Soc. Opt. Eng. 4963, 259-260.

  24. Wallrabe, H., Elangovan, M., Burchard, A., Periasamy, A. and Barroso, M. (2002) FRET microscopy reveals clustered distribution of co-internalized receptor-ligand complexes in the apical recyling endosome of polarized epithelial MDCK cells. Proc. SPIE Int. Soc. Opt. Eng. 4620, 64-73.

  25. Elangovan, M., Day, R.N. and Periasamy, A. (2002) FRET-FLIM microscopy. Proc. SPIE Int. Soc. Opt. Eng. 4620, 267-273.

  26. Kneas, K.A., Demas, J.N., DeGraff, B.A. and Periasamy, A. (2001) Comparison of conventional, confocal, and two-photon microscopy for detection of microcrystals within luminescence-based oxygen sensor films (Invited Paper). Proc. SPIE Int. Soc. Opt. Eng. 4262: 89-97.

  27. Day, R.N. and Periasamy, A. (2001) FRET microscopy to visualize transcription factor dimerization in the nucleus of the living cell (Invited Paper). Proc. SPIE Int. Soc. Opt. Eng. 4262: 163-170. 

  28. Elangovan, M. and Periasamy, A. (2001) Bleed-hrough and photobleaching correction in multiphoton FRET microscopy. Proc. SPIE Int. Soc. Opt. Eng.  4262: 177-185. 

  29. Ganesan, S., Elangovan, M. and Periasamy, A. (2001) Kinetics and comparison of d-aminolevulinic-acid-induced endogenous protoporphyrin-IX in single cell by steady state and multiphoton fluorescence imaging. Proc. SPIE Int. Soc. Opt. Eng. 4262: 263-267.

  30. Abraham, A.T., Brautigan, D.L., Hecht, S.M. and Periasamy, A. (2001) Localization of bleomycin in a single living cell using three-photon excitation microscopy. Proc. SPIE Int. Soc. Opt. Eng. 4262: 347-353. 

  31. Periasamy, A., Elangovan, M. and Day, R.N. (2001) Comparison of one- and two-photon fluorescence resonance energy transfer microscopy. Proc. SPIE Int. Soc. Opt. Eng. 4262: 366-372. 

  32. Wallrabe, H., Elangovan, M., Periasamy, A. and Barroso, M., (2001) Use of laser scanning confocal and two-photon FRET microscopy to image and quantify the colocalization of fluorophore-labeled ligands in MDCK epithelial cells. Proc. SPIE Int. Soc. Opt. Eng. 4262: 373-382.

  33. Noakes, C.R., Goto, T., Keller, R. and Periasamy, A. (2001) Does digital deconvolution improve two-photon microscopy in deep tissue imaging? Proc. SPIE Int. Soc. Opt. Eng. 4262: 389-395.

  34. Periasamy, A. (2000) Two-photon energy transfer microscopy. Proc. SPIE, 3921, 299-304.

  35. Elangovan, M., Peter, K. and Periasamy, A. (2000) Fluorescence lifetime imaging: Simulation and error analysis. Proc. SPIE Int. Soc. Opt. Eng. 3921: 280-287. 

  36. Periasamy, A., Sharman, K.K., Ahuja, R. Eto, I. and Brautigan, D.L. (1999) Fluorescence Lifetime Imaging of Green Fluorescent Protein of Living Cells. Proc. SPIE Int. Soc. Opt. Eng. 3604: 6-12.

  37. Periasamy, A. (1999) Multi-photon excitation fluorescence microscopy in the biomedical sciences. Proc. SPIE Int. Soc. Opt. Eng.  3604: 74-82.

  38. Periasamy, A. (1998) Digital deconvolution FRET microscopy: 3D visualization of protein-protein interactions in a single living cell. Proc. SPIE Int. Soc. Opt. Eng. 3260: 106-114.

  39. Periasamy, A., Kay, S.A. and Day, R.N. (1997) Fluorescence resonance energy transfer (FRET) imaging of a single living cell using green fluorescent protein Proc. SPIE Int. Soc. Opt. Eng. 2983: 58-66.

  40. Periasamy, A., and Day, R. N. (1997) Pit-1 protein localization at different optical sections in a single living cell using FRET microscopy and green fluorescent proteins. In “Proc. Microscopy and Microanalysis” (G. W. Bailey, R. V. W. Dimlich, K. B. Alexander, J. J. McCarthy, T. P. Pretlow, Eds.), Vol. 3 pp. 133-134. Springer-Verlag, New York. 

  41. Herman, B., Wang, X.F., Periasamy, A., Kwon, S., Gordon, G.W., Wodnicki, P. (1996) Fluorescence lifetime imaging in cell biology. Proc. SPIE Int. Soc. Opt. Eng. 2678: 88-97.

  42. Wang, X.F., Periasamy, A., Gordon, G.W., Wodnicki, P. and Herman, B. (1994) Fluorescence lifetime imaging microscopy (FLIM) and its applications. Proc. SPIE Int. Soc. Opt. Eng. 2137: 64-76.

  43. Diliberto, P.A., Periasamy, A. and Herman, A. (1991) Distinct oscillations in cytosolic and nuclear free calcium in single intact living cells demonstrated by confocal microscopy. In: Proc: 49th Ann. Meet. Elect. Micros. Soc. America (EMSA). Ed: G.W.Bailey, San Francisco Press Inc., San Francisco, pp228-229.

  44. Periasamy, A., Lindroth, J. R. and Thompson, R. P. (1988) Real time analysis of video image and electrocardiogram of the chick embryo heart. In: Proc. of the Ann. Int. Conf. IEEE/EMBS, New Orleans, 10: 483-484.

  45. Periasamy, A., Holdren, D. N., Everts, W. C. and Pollack, G. H. (1985) Digital image processing of muscle striations. In:Proc. Annual Int. Conf. IEEE/EMBS, Chicago, 7: 971-975.

  46. Burns, D. H., Holdren, D. N., Periasamy, A., Everts, W. C. and Pollack, G. H. (1985) Image processing of muscle striations below the resolution limit of the microscope. Medical Image Processing/SPIE-593: 46-51.

  47. Ramachandran, G., Periasamy, A. and Singh, M. (1985) Laser speckle displacement cardiography. in:Proc. Annual Int. Conf.IEEE/EMBS, Chicago, 7: 1192-1195.

  48. Burns, D.H., Holdren, D.N., Periasamy, A., Everts, W.C. and Pollack, G.H. (1985) Image processing of muscle striations below the resolution limit of the microscope. Medical image Proc. SPIE Int. Soc. Opt. Eng. 593: 46-51.

  49. Sridharan, K. S., Mothi S. F.., Rajalakshmi, M. G., Natarajan, S. and Periasamy, A. (1984) A learner's aid for studies on bioelectronics. in:Proc. Int. Conf. on Teaching Aids in Phy. Education (TAPE'84), Madras, p24-28.