Second Harmonic (SHG) and Third Harmonic Generation (THG) Imaging

We can image SHG using the Zeiss 780 and Zeiss 510 multiphoton microscopy systems. The THG & SHG can be imaged using the Zeiss 780 multiphoton system...

Biomarkers (NAD(P)H, NADH, FAD, Tryptophan) for Cellular Metabolism

The importance of autofluorescence for studying cells and tissues lies mainly in its potential for diagnostic applications and as a research tool for understanding the underlying mechanisms of molecular interactions and cellular processes under native conditions...

Intravital/In vivo Imaging Microscopy

The Zeiss 780 multiphoton system equipped with intravital imaging option (Objective converter). The existing microscope is an inverted epi-fluorescent microscope and we have included an objective inverter for intravital imaging...

Fluorescence Lifetime Imaging Microscopy (FLIM)

The fluorescence lifetime refers to the average time the molecule stays in its excited state before emitting a photon, which is an intrinsic property of a fluorophore...

Fluorescence Correlation and Cross-Correlation Spectroscopy (FCS)

Two systems are available at the Keck Center for FCS measurements - Becker & Hickl FLIM system coupled to the Zeiss 780 with two-photon illumination; ISS Alba system with one-photon illumination...

High Content Screening System (multiwell plates)

This is a custom developed system based on the existing wide-field microscopy system. Two color High-content screening of multi-well plates (Max 96 well plates)...

Förster (or fluorescence) resonance energy transfer (FRET) Microscopy

FRET is a process by which radiationless transfer of energy occurs from a fluorophore molecule in the excited state to a molecule in close proximity in the ground state...