An Evaluation of Two-Photon Excitation Versus Confocal and Digital Deconvolution Fluoescence Microscopy Imaging in Xenopus Morphogenesis
by Ammasi Periasamy
ABSTRACT: The ability to visualize cell motility occurring deep in the context of opaque tissues will allow many currently intractable issues in developmental biology and organogenesis to be addressed. In this study, we compare two-photon excitation with laser scanning confocal and conventional digital deconvolution fluorescence microscopy, using the same optical configuration, for their ability to resolve cell shape deep in Xenopus gastrula and neurula tissues....
Microscopy Research and Technique 47:172-181 (1999)
Fluorescence Microscopy Study Of Heterogeneity In Polymer-Supported Luminescence-Based Oxygen Sensors
by Kristi A. Kneas
ABSTRACT: Despite the great potential of fluorescence microscopy, its application to date has largely been in the study of biological specimens. It will be shown that conventional fluorescence microscopy provides an invaluable tool with which to study the photophysics of polymer-supported luminescence-based oxygen sensors....
Microsc. Microanal. 6, 551-561, 2000
Characterization of Two-photon Excitation Fluorescence Lifetime Imaging Microscopy for Protein Localization
YE CHEN AND AMMASI PERIASAMY
ABSTRACT Two-photon excitation .uorescence resonance energy transfer (2P-FRET) imaging microscopy can provide details of speci.c protein molecule interactions inside living cells. Fluorophore molecules used for 2P-FRET imaging have characteristic absorption and emission spectra that introduce spectral cross-talk (bleed-through) in the FRET signal that should be removed in the 2P-FRET images, to establish that ........
MICROSCOPY RESEARCH AND TECHNIQUE 63:72–80 (2004)