List of Publications

Ammasi Periasamy, Ph.D.

Complete List of Publications

 

Books

“FRET Microscopy: Critical Discussions on Image Acquisition and Analysis” Cambridge University Press, 2015. In Preparation.

“FLIM Microscopy in Biology and Medicine” CRC Press (Taylot & Francis Group), pages 407, 2010

“Molecular Imaging:FRET Microscopy and Spectroscopy” Oxford University Press, NY, pages 312, 2005.

 “Methods in Cellular Imaging” Oxford University Press, NY, pages434, 2001. 

(Abstracts are NOT included) (Total 151)

 

Peer-reviewed Journals (81)

  1. Sun, Y. and Periasamy, A. (2015) Localizing protein-protein interactions in living cells using fluorescence lifetime imaging microscopy. Methods in Mol. Biol., Vol. 1251: 83-108.
  2. Wallrabe, H., Sun, Y., Fang, X., Periasamy A. and Bloom, G.S., (2015) Three-color confocal FRET microscopy: quantitative analysis of protein interactions in the nucleation of actin filaments in live cells. Cytometry A., In Press.
  3. Zaman, K., et al.,  Periasamy, A., Lewis, S.J., and Gaston, B. (2015) Augmentation of CFTR maturation by S-nitrosoglutathione reductase. PLoS ONE, under revision.
  4. Burdikova, Z., Svindrych, Z., Pala, J., Hickey, C., Wilkinson, M.G., Panek, J., Auty, M.A.E.,  Periasamy, A. Sheehan, J.J.,  (2015) Measurement of pH micro-heterogeneity in natural cheese matrices by fluorescence lifetime imaging, Frontiers in Microbiology, 6: 00183.
  5. Rehman, S., Gladman, J.T., Periasamy, A., Sun, Y. and Mahadevan, M.S. (2014) Development of an AP-FRET based analysis for characterinzing RNA-protein interactions in myotonic dystrophy (DM1). PLoS ONE 9(4):e95957. PMCID: PMC4004549.
  6. Wallrabe, H., Periasamy, A. and Elangovan, M. (2014) Microscopy core facilities: Results of an international survey. Microscopy Today March: pp36-44.
  7. Jyothikumar, V., Sun, Y. and Periasamy, A. (2013) Investigation of tryptophan-NADH in live human cells using 3-photon fluorescence lifetime imaging. J. Biomed. Opt. 18(6): 060501. PMCID: PMC3675329.
  8. Sun, Y., Rombola, C., Jyothikumar, V. and Periasamy, A. (2013) Förster resonance energy transfer microscopy and spectroscopy to localize protein-protein interactions in live cells. Cytometry A. 83A(9): 780-793.
  9. Wallrabe, H., Y. Cai, Y. Sun,  A. Periasamy, R. Luzes, X. Fang, H.-M. Kan, L.C. Cameron, D.A. Schafer and G.S. Bloom, (2013) “IQGAP1 interactome analysis by In Vitro reconstitution and live cell 3-color FRET microscopy”, Cytoskeleton, 70: 819-836. PMCID: PMC3917506.
  10. Ken Abe, Lingling Zhao,  Ammasi Periasamy, Xavier Intes, Margarida Barroso (2013) Non-Invasive In Vivo Imaging of Breast Cancer Cell Internalization of Transferrin by Near Infrared FRET. PLoS ONE 8(11): e80269. PMCID: PMC3836976.
  11. Sun, Y. Hays, N.M., Periasamy, A., Davidson, M.W. and Day, R.N. (2012) Monitoring protein interactions in living cells with fluorescence lifetime imaging microscopy. Meth. Enzymol., 504: 371-391. PMCID: PMC4136481.
  12. Williams, M., Burdsal, C., Periasamy, A. and Lewandoski, M. and Sutherland, A. (2012) Mouse primitive strek forms in situ by initiation of epithelial to mesenchymal transition without migration of cell population. Dev. Dynamics 241(2):270-283. PMCID: PMC3266444.
  13. Sun, Y., Day, R.N. and Periasamy, A. (2011) Investigating protein-protein interactions in living cells using fluorescence lifetime imaging microscopy. Nature Protocols 6: 1324-1340. PMCID: PMC3169422.
  14. Sun, Y., Wallrabe, H., Seo, S.-A. and Periasamy, A. (2011) FRET Microscopy in 2010: The Legacy of Theodor Förster on the 100th Anniversary of his Birth. ChemPhysChem,12:462-474. PMCID: PMC3422661.
  15. Sun, Y., Wallrabe, H. Booker, C., Day, R.N. and Periasamy, A. (2010) Three-color spectral FRET microscopy localizes three interacting proteins in living cells. Biophysical J. Vol. 99, 1274-1283. PMCID: PMC2920763
  16. Li, H, Yu P, Sun Y, Felder RA, Periasamy A, and Jose PA. (2010) Actin cytoskeleton-dependent Rab GTPase-regulated angiotensin type I receptor lysosomal degradation studied by fluorescence lifetime imaging microscopy. J Biomed Optics. 15(5): 056003 (pp1-9). PMCID: PMC2966490
  17. Sun, Y., and Periasamy, A. (2010) Additional correction for energy Transfer efficiency calculation in filter-based Förster resonance energy transfer microscopy for more accurate result. J Biomed. Opt. 15(2): 020513 (pp1-3). PMCID: PMC2874045
  18. Sun, Y. Booker, C.F., Kumari, S., Day, R.N., Davdison, M. and Periasamy, A. (2009) Characterization of an Orange Acceptor Fluorescent Protein for Sensitized Spectral FRET Microscopy using a White Light Laser. J. Biomed. Opt. 14(5), 054009. PMCID: PMC2774974
  19. Yen, W., Williams, M., Periasamy, A., Conaway, M., Burdsal, C., Keller, R., Lu, X., and Sutherland, A. (2009) PTK7 is essential for polarized cell motility and convergent extension during mouse gastrulation. Development 136: 2039-2048. PMCID: PMC2685725
  20. Periasamy, A., Wallrabe, H. and Barroso, M. (2009) Quantitation of protein-protein interactions: Confocal FRET Microscopy. Meth. Cell Biol. 89: 569-598. PMCID: PMC2685725
  21. Hewang Li et al., Periasamy, A. et al. and Pedro Jose. (2008) Dopamine 5 receptor mediates Ang II type 1 receptor degradation via a ubiquitinproteasome pathway in mice and human cells. J Clin Invest. 118: 2180-2189. PMCID: PMC2373421
  22. Day, R.N., Booker, C. and Periasamy, A. (2008) The Characterization of an improved donor fluorescent protein for Förster resonance energy transfer microscopy. J. Biomed. Opt. 13: 031203 (pp1-9). PMCID: PMC2483694
  23. Li, H., Li, Hui-Fang, Felder, R.,  Periasamy, A. and Jose, P.A. (2008) Rab4 and Rab11 coordinately regulate the recycling of angiotensin II type I receptor as demonstrated by fluorescence resonance energy transfer microscopy J. Biomed. Opt. 13: 031206 (pp1-10). PMCID: PMC3731076
  24. Chen, Y. J.P. Mauldin, R.N. Day and Periasamy, A. (2007) Characterization of spectral FRET imaging microscopy for monitoring the nuclear protein interactions. J Microscopy, 228:139-152. PMCID: PMC2874973
  25. Wallrabe, H., Bonamy, G., Periasamy, A. and Barroso, M. (2007) Receptor complexes co-transported via polarized endocytic pathways form clusters with distinct organizations. Mol. Biol. Cell. 18:2226-2243. PMCID: PMC1877110
  26. Mikhailova, M.V., Mayeux, P.R., Jurkevich, A., Kuenzel, W.J., Madison, F., Chen, Y, Periasamy, A. and Cornett, L.E. (2007) Hetero-Oligomerization between Vasotocin and Corticotropin-Releasing Hormone (CRH) Receptors Augments CRH-Stimulated Cyclic AMP Production. Molecular Endocrinology. 21: 2178-2188.
  27. Ren, Q.-X., Douglass III, V., Xu, W., Sabat, M., Periasamy, A. Demas, J.N. and DeGraff, B.A. (2007) Potassium ion induced changes of crystal structure and fluorescence of a crpwn ether. J Fluorescence.
  28. Chen, Y. and Periasamy, A. (2007) Dynamic imaging of protein-protien interactions in living cells using confocal and spectral imaging FRET microscopy. Indian J Exp. Biol., 45:48-57.
  29. Demarco, I. A., Periasamy, A., Booker, C. F. and Day, R. N. (2006) Monitoring dynamic protein interactions with photo-quenching FRET. Nature Methods 3(7):519-524. PMCID: PMC2921620
  30. Abadir,, P.M., Periasamy, A., Carey, R.M. and Siragy, H.M. (2006) Angiotension II type 2 receptor-Bradykinin B2 receptor functional heterodimerization. Hypertension (JAHA, Journal of the American Heart Association) 48:1-7.
  31. Chen, Y. and Periasamy, A. (2006) Intensity range based quantitative FRET data analysis to localize the protein molecules in living cell nucleus. J. Fluorescence. 16:95-104.
  32. Wallrabe, H. Chen, Y., Periasamy, A. And Barroso, M. (2006) Issues in confocal microscopy for quantitative FRET analysis. Microscopy research and Techniques. 69:196-206.
  33. Wallrabe, H., and Periasamy, A. (2005) Imaging Protein molecules using FRET-FLIM microscopy. Current Opinion in Biotechnology. 16:19-27.
  34. Periasamy, A. and Chen, Y. (2005) Quantitative Analysis of Protein-Protein Interactions: One- and Two-photon Excitation FRET-FLIM Microscopy. Microscopy and Microanalysis. 11(suppl 2):12-13.
  35. Chen, Y. and Periasamy, A. (2004) Characterization of two-photon excitation fluorescence lifetime imaging microscopy for protein localization. Microscopy Research and Techniques. 63:72-80.
  36. Day, R.N., Demarco, I.A., Voss, T.C., Chen, Y. and Periasamy, A. (2004) FLIM-FRET microscopy to visualize transcription factor interactions in the nucleus of the living cell. Progress in Biomedical Optics and Imaging, 5323:36-43.
  37. Chen, Y., Mills, J.D. and Periasamy, A. (2003) Protein interactions in cells and tissues using FLIM and FRET. Differentiation. 71:528-541.
  38. Sekar, R.B. and Periasamy, A. (2003)  Fluorescence resonance energy transfer (FRET)  microscopy imaging of live cell protein localization. J. Cell Biol., 160:629-633. PMCID: PMC2173363
  39. Solenski, N.J., Kostecki, V.K., Dovery, S. and Periasamy, A. (2003) Nitric-oxide-induced depolarization of neuronal mitochondria:implications for neuronal cell death. Molecular and Cellular Neuroscience. 24:1151-1169.
  40. Day, R.N., Voss, T.C., Enwright III, J.F., Booker, C.F., Periasamy, A. and Schaufels, F. (2003) Imaging the localized protein interactions between Pit-1 and the CCAAT/enhancer binding protein alpha (C/EBPa) in the living pituitary cell nucleus. Mol. Endo. 17(3), 333-345.
  41. Elangovan, M., Wallrabe, H., Chen, Y., Day, R.N., Barroso, M. and Periasamy, A. (2003) Characterization of one- and two-photon excitation resonance energy transfer microscopy. Methods. 29, 58-73.
  42. Wallrabe, H., Elangovan, M., Burchard, A., Periasamy, A. and Barroso, M. (2003) Confocal FRET microscopy to measure clustering of receptor-ligand complexes in endocytic membranes. Biophysical J. 85:559-571. PMCID: PMC1303111
  43. Mills, J.D., Stone, J.R., Rubin, J.D., Melon, D.E., Okonkwo, D.O., Periasamy, A. and Helm, G.A. (2003) Illuminating Protein Interactions in Tissue using Confocal and Two-photon Excitation Fluorescent Resonance Energy Transfer (FRET) Microscopy. J. Biomed. Opt. 8: 347-356.
  44. Wallrabe, H., Stanley, M., Periasamy, A. and Barroso, M. (2003) One- and two-photon fluorescence resonance energy transfer microscopy to establish a clustered distribution of receptor-ligand complexes in endocytic membranes. J Biomed. Opt. Vo. 8(3), 339-346.
  45. Bowman, R.D., Kneas, K., Demas, J.N. and Periasamy, A. (2003) Conventional, confocal, and two-photon fluoresecnce microscopy investigations of polymer-supported oxygen sensors. J. Microscopy 211:112-120.
  46. Elangovan, M., Day, R.N. and Periasamy, A. (2002) Dynamic imaging using fluorescence resonance energy transfer. Biotechniques 32, 1260-1265.
  47. Elangovan, M., Day, R.N. and Periasamy, A. (2002) Nanosecond fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy to localize the protein interactions in a single living cell. J Microscopy. 205:3-14.
  48. Gale, J.E., Meyers, J.R., Periasamy, A. and Corwin, J.T. (2002) Survival of bundleless hair cells and subsequent bundle replacement in the bullfrog’s saccule. J. Neurobiol.50, 81-92.
  49. Periasamy, A. (2001) Fluorescence resonance energy transfer microscopy:a mini review. J Biomed. Opt. 6: 287-291.
  50. Day, R.N., Periasamy, A. and Schaufele, F. (2001) Fluorescence resonance energy transfer microscopy of localized protein interactions in the living cell nucleus. Methods. 25: 4-18.
  51. Periasamy A, Noakes C, Elangovan M, Keller R, Day R. (2001) MultiPhoton Microscopy and its Applications in the Biomedical Sciences.  The Spectrum, Bowling Green University. 14:3: 13-19.
  52. Kneas, K. Demas, J., DeGraff, B.A. and Periasamy, A. (2000) Fluorescence microscopy study of heterogeneity in polymer-supported luminescence-based oxygen sensors. Microscopy and Microanalysis. 6: 551-556.
  53. Resnick, E.M., Schreihofer, D.A., Periasamy, A. and Shupnik, M.A. (2000) Truncated estrogenreceptor product-1 suppresses estrogen receptor transactivation by dimerization with estrogen receptors a and b. J. Biol. Chem., 275: 7158-7166.
  54. Stenz, M. and Periasamy, A. (2000) Fluorescence resonance energy transfer microscopy. Microscopy Today, issue#00-3 (April), 10-12.
  55. Abraham, A.T., Periasamy, A., Brautigan, D.L. and Hecht, S.M. (2000) Localization of bleomycin in mammalian cells utilizing fluorescence microscopy. J. Natural Products. Cover Page, January –April issues.
  56. Chen, S-yu., Periasamy, A., Bing, Y. Herman, B., Jacobson, K. and Sulik, K.K. (2000) Differential sensitivity of mouse neural crest cells to ethanol-induced toxicity. Alcohol 20: 75-81.
  57. Periasamy, A., Skoglund, P., Noakes, C. and Keller, R. (1999) An evaluation of two-photon excitation versus confocal and digital deconvolution fluorescence microscopy imaging in Xenopus morphogenesis. Micros. Res. Tech. 47, 172-181.
  58. Sharman, K. K., Periasamy, A., Asworth, H., and Demas, J. N. (1999) Error analysis of the rapid lifetime determination method for double-exponential decays and new windowing schemes. Anal. Chem., 71: 947-952.
  59. Periasamy, A. and Day, R.N. (1999) Visualizing Protein Interactions in Living Cells using Digitized GFP Imaging and FRET Microscopy. Meth. Cell Biol., 58: 293-314.
  60. Periasamy, A. and Day, R. N. (1998) FRET imaging of Pit-1 protein interactions in living cells. J. Biomed.Opt. 3: 154-160.
  61. Herman, B., Wodnicki, P., Kwon, S., Periasamy, A., Gordon, G.W., Mahajan, N., and Wang, X.F. (1997) Recent developments in monitoring calcium and protein interactions in cells using fluorescence lifetime microscopy. J. Fluores., 7: 85-91.
  62. Periasamy, A., Wodnicki, P., Wang, X.F., Kwon, S., Gordon, G.W. and Herman, B.  (1996) Time-resolved fluorescence lifetime imaging microscopy using a picosecond pulsed tunable dye laser system. Rev. Sci. Instrum. 67: 3722-3731.
  63. Periasamy, A., Wang, X.F., Wodnicki, P., Gordon, G.W., Kwon, S., Diliberto, P.A.  and Herman, B. (1995) High speed fluorescence microscopy: Lifetime imaging in the biomedical sciences.  J. Micros. Soc. Am., 1: 13-23.
  64. Periasamy, A., Siadat-Pajouh, M., Wodnicki, P., Wang, X.F. and Herman, B.  (1995) Time gated fluorescence microscopy in clinical imaging.  Micros. Anal., (March) 33-35. 
  65. Periasamy, A. and Herman, B. (1994) Computerized fluorescence microscopic vision in biomedical sciences. J. Comp. Assist. Micros., 6: 1-26.
  66. Siadat-Pajouh, M., Periasamy, A., Ayscue, A.H., Moscicki, A.B., Palefsky, J.M., Walton, L.,DeMars, L.R., Power, J.D., Herman, B., and Lockett, S.J.  (1994) Detection of human papillomavirus type 16/18 DNA in cervicovaginal cells by fluorescence based in situ hybridization and automated image cytometry. Cytometry 15: 245-257.
  67. Siadat-Pajouh, M., Ayscue, A.H., Periasamy, A. and Herman, B.  (1994) Introduction of fast and sensitive fluorescent in situ hybridization method for single copy: Detection of human papillomavirus (HPV) genome.  J. Histochem. Cytochem. 42: 1503-1512.
  68. Wang, X. F., Periasamy, A., Coleman, D. M. and Herman, B.  (1992) Fluorescence lifetime imaging microscopy: Instrumentation and Applications.  Critical Rev. Analyt. Chem., 23(5): 369-395.
  69. Periasamy, A., Lindroth, J.R. and Thompson, R.P.  (1992) Development of a computer-aided video imaging system to study the embryonic chick heart in situ.  Innov. Tech. Med. Biol.,  13: 30-45.
  70. Shoji, Y., Fisher, M.H., Periasamy, A., Herman, B. and Juliano, R.L.  (1991) Verapamil and Cycolsporin a modulate Doxorubicin Toxicitin.  Cancer Letters. 57:  209-218.
  71. Shoji, Y., Akhtar, S., Periasamy, A., Herman, B. and Juliano, R.L.  (1991) Mechanism of cellular uptake of modified oligodeoxynucleotides containing methylphosphonate linkages.  Nucleic Acid Res. 19: 5543-5580.
  72. Chen, E.Q., Lam, C.F. and Periasamy, A.  (1991) Effect of refraction on optical microscopic measurement of internal blood-vessel diameter and its correction.  J. Micros. 164: 239-245.
  73. Periasamy, A. and Singh, M. (1991) Analysis of cardiac displacement pattern as observed on the chest wall by time-average speckle interferometry in health and diseases.  Innov. Tech. Med. Biol., 12: 614-638.
  74. Periasamy, A., Burns, D.H., Holdren, D. N., Pollack, G. H., and Trombitas, K. (1990) A-Band shortening in single fibers of frog skeletal muscle.  Biophys. J., 57: 815-828. PMCID: PMC1280782
  75. Pollack, G.H., Granzier, H.L.M., Mattiazzi, A., Trombitas, K., Periasamy, A. et al  (1988)  Pauses, steps, and the mechanism of contraction.  Adv. Exp. Med. Biol. 226: 617-642.
  76. Singh, M., Ramachandran, G. and Periasamy, A. (1986) Laser speckle displacement cardiography. J. Opt., 15: 63-66.
  77. Periasamy, A. and Singh, M. (1985) Reconstruction of cardiac displacement patterns on the chest wall by laser speckle interferometry. IEEE Trans. Med. Imaging.,MI-4: 52-57.
  78. Singh, M. and Periasamy, A. (1984) Determination of safe exposure levels of He/Ne laser light to the human body by means of erythrocyte irradiation studies. Med. Biol. Engg. Comput., 22: 147-150.
  79. Singh, M., Periasamy, A. and Chitra, T. (1982) Development of an optical fiber technique for He-Ne laser screening of human body and its comparison with the integrating sphere method. Med. Biol. Engg. Comput., 20: 111-112.
  80. Periasamy, A., Singh, M. and Sivaram, B. M. (1981) Detection of human cardiac activity at apex region by laser speckle. Curr. Sci., 50: 302-304.
  81. Periasamy, A., Sivaram, B. M. and Singh, M. and Meyyappan, A. (1979) Measurement of displacement of a curved surface using laser speckle. J. Opt., 8: 12-19.

Book Chapters (25)

  1. Periasamy, A. Mazumdar, N., Christopher, K., Sun, Y., and Day, R.N. (2015) Förster resonance enrgy transfer technique by lifetime measurement. In “Advanced Time-Correlated Single Photon Counting Applications” Ed. Wolfgang Becker, Springer, In Press.
  2. Jyothikumar, V., Sun, Y., and Periasamy, A. (2015) Förster resonance energy transfer microscopy for monitoring molecular dynamics in living cells. In “Fundamentals of Fluorescence Imaging”, ed by Guy Cox., 2014. submitted.
  3. Jyothikumar, V., Sun, Y., and Periasamy, A. (2015) Tryptophan as an alternative biomarker for Cellular Metabolism. In “Natural Biomarkers for Cellular Metabolsm; Biology, Techniues, and Applications”. Eds. Ghukasyan, V. and Heikal, A. CRC Press (Francis & Taylor group). (Series in Cellular and Clinical Imaging, Serious Editor: Ammasi Periasamy). Pp 157-177.
  4. Sun, Y. and Periasamy, A. (2013) Fluorescence Microscopy Imaging. In “Biomedical Optical Imaging Technologies: Design and Applications”, Ed. Rongguang Liang, Springer, pp79-110.
  5. Barroso, M., Sun, Y., Wallrabe, H. and Periasamy, A. (2013) Nanometer-scale measurements using FRET and FLIM microscopy. In: Luminescence: the Instrumental key to the future Nanotechnology, ed by Adam Gilmore, Pan Stanford Publishers, NY. In Press.
  6. Day, R.N., Periasamy, A. and Demarco, I. (2010) Imaging protein interactions in living cells using the fluorescent proteins. In: Fluorescent Proteins and their uses. Topics in Fluorescence Spectroscopy 2008, Vol. 5. Eds. C.D. Geddes, Springer-Verlag, NY. Pp 337-358.
  7. Sun, Y., Demas, J.N. and Periasamy, A. (2009) Wide-field fluorescent lifetime imaging microscopy using gated image intensifier. In FLIM Microscopy in Biology and Medicine, Eds. A. Periasamy and R.M. Clegg, Taylor & Francis-CRC Press. Pp. 93-115.
  8. Periasamy, A. and Clegg, R.M. (2009) FLIM applications in biomedical sciences. In FLIM Microscopy in Biology and Medicine, Eds. A. Periasamy and R.M. Clegg, Taylor & Francis-CRC Press. Pp. 385-400.
  9. Merchant, F. and Periasamy, A. (2008) Fluorescence imaging. In: “Microscope Image Processing” Eds. Q. Wu, F. Merchant and K.R. Castleman, Elsevier, NY. Pp. 247-297.
  10. Chen, Y., Demarco, I.A., Booker, C.F., and Day, R.N. (2009) Localization of protein-protein interactions in living cells: FRET Microscopy. In: “Methods in Molecular Biology-Fluorescent Proteins: Methods and Applications”, Ed. J. Rothnagel. Humana Press.
  11. Chen, Y., Wallrabe, H. and Periasamy, A. (2007) Two-photon Förster resonance energy transfer (FRET) microscopy. In: Handbook of Biomedical Nonlinear Optical Microscopy. Eds. B.R. Masters and P.T.C. So, Oxford University Press-New York. Pp599-615.
  12. Periasamy, A. (2006) Cellular Imaging. In: Encyclopedia of Medical Devices and Instrumentation, (John G. Webster, ed.) Hoboken: John Wiley & Sons, Inc. NY. 2nd edition, Vol. 2, pp. 90-101.
  13. Mills, J.D., Stone, J.R., Okonkwo, O.D., Periasamy, A., and Helm, G.A. (2005) Multiphoton FRET microscopy for protein localization in tissue. In: Molecular Imaging:FRET Microscopy and Spectroscopy. Eds. A. Periasamy and R. N. Day, Oxford University Press-New York. Chapter 6. 112-125.
  14. Chen, Y., Elangovan, M. and Periasamy, A. (2005) FRET data Analysis-The algorithm. In: Molecular Imaging:FRET Microscopy and Spectroscopy. Eds. A. Periasamy and R. N. Day, Oxford University Press-New York. Chapter 7. 126-145.
  15. Chen, Y. and Periasamy, A. (2005) Time correlated single photon counting (TCSPC) FLIM-FRET microscopy for protein localization. In: Molecular Imaging:FRET Microscopy and Spectroscopy. Eds. A. Periasamy and R. N. Day, Oxford University Press-New York. Chapter 13. 239-259.
  16. Periasamy, A., Noakes, C., Skoglund, P., Keller, R. and Sutherland, A.E. (2002) Two-photon excitation fluorescence microscopy imaging in Xenopus and transgenic mouse embryos. In: “Confocal and Two-photon Microscopy:Foundations, Applications and Advances”, Ed. A. Diaspro, Wiley-Liss, Inc, NY. pp 271-283.
  17. Periasamy, A., Elangovan, M., Elliott, E. and Brautigan, D.L. (2002) Fluorescence lifetime imaging of green fluorescent fusion proteins in living cell. In: “Methods in Molecular Biology, Vol. 183, Green Fluorescent Protein: Applications and Protocols”, Ed. B. Hicks., Humana Press, NJ. pp. 89-100.
  18. Noakes, H. and Periasamy, A. (2001) Processing microscope acquired-images for use in multimedia, print and the Worl Wide Web. In: “Methods in Cellular Imaging”, Ed. A. Periasamy, Oxford Univ. Press, NY, pp 128-142.
  19. Periasamy, A., Elangovan, M, Wallrabe, H., Demas, J.N., Barroso, M., Brautigan, D.L. and Day, R.N. (2001) Wide-field, Confocal, two-photon and Lifetime  resonance Energy Transfer Imaging Microscopy. In: “Methods in Cellular Imaging”, Ed. A. Periasamy, Oxford University Press, NY. Pp295-308.
  20. Herman, B., Wang, X.F., Wodnicki, P., Periasamy, A., Mahajan, N., Berry, G. and Gordon, G. (1999) Fluorescence lifetime imaging microscopy. In: Applied Fluorescence in Chemistry, Biology and Medicine. (Eds. B. Strehmel, S. Schrader, H. Seifert and W. Rettig) Springer-Verlag, New York. pp491-507.
  21. Wang, X.F., Periasamy, A., Wodnicki, P., Gordon, G.W., and Herman, B. (1996) “Time resolved Fluorescence Lifetime Imaging Microscopy: Instrumentation and Biomedical Applications”, In: Fluorescence Imaging Spectroscopy and Microscopy, Eds. X. F. Wang and B. Herman, Chemical Analysis Series, Vol.137, Wiley & Sons, New York, p 313-350.
  22. Periasamy, A. and Singh, M. (1985) Detection of cardiac abnormalities by laser speckle and its comparison with 2D-echocardiography. in: Physiological Fluid Dynamics-1, Eds.S. D. Nigam and M. Singh, SCPFD Publications, I.I.T., Madras, p193-198.
  23. Sivaram, B. M. and Periasamy, A. (1985) Velocity profiles of fluid flow by laser speckle interferometry. in: Physiological Fluid Dynamics-1, Eds. S. D. Nigam and M. Singh, SCPFD Publications, I.I.T., Madras, p277-280.
  24. Sivaram, B. M., Hemachandran, N., Periasamy, A., and Palanisamy, P. K. (1983) Determination of orthodontic centres of rotation and axes of rotation by laser speckle interferometry. Biomed. Engg. II, Ed. C. William Hall, Pergamon, p17-20.
  25. Periasamy, A., Singh, M. and Kalyani, N. (1983) Identification of the cardiac abnormalities by laser speckle pattern as observed on the chest wall. Biomed. Eng. II, Ed. C. William Hall, Pergamon Press, 393-396.

Proceedings (45)

  1. Rehman, S., Brown-Steinke, K., Palmer, L., Periasamy, A. (2015) Confocal immunofluorescence FRET microscopy to investigate eNOS and GSNOR localization and interaction in pulmonary endothelial cells. SPIE Proc. 9329: In Press.
  2. Wallrabe, H., Svindrych, Z. and Periasamy, A. (2015) Comprehensive quantitative evaluation of FLIM-FRET image data using intensity-based analysis tools. SPIE Proc. 9329:  In Press.
  3. Sun, Y. and Periasamy, A. (2012) Monitoring Protein-Protein Interactions in Living Specimens using FLIM-FRET Microscopy. Proc. Microsc. Microanal. 18 (Suppl2):148-149.
  4. Sun, Y., Wang, L., Jyothikumar, V., Brautigan, D. B. and Periasamy, A. (2012) Characterizing the Interactions between Prolyl Isomerase Pin1 and Phosphatase Inhibitor-2 in Living Cells with FRET and FCS. SPIE Proc. 8226: pp.82263T-(1-9).
  5. Horst, W., Sun, Y., Fang, X., Periasamy, A. and Bloom, G. (2012) Three-color FRET expands the ability to quantiy the interactions of several proteins involve in actin filament nucleation. SPIE Proc. 8226: pp.82260J-(1-10).
  6. Jyothikumar, V., Sun, Y. and Periasamy, A. (2012) Monitoring changes in Endogenous fluorophores through quantitative FLIM imaging in live cells. SPIE Proc. 822: pp. 822644.
  7. Sun, Y. Seo, S., Provence, S. and Periasamy, A. (2010) Comparison of FRET microscopy imaging techniques for studying protein-protein interactions in living cells using FRET standards. SPIE Proc. 7569.
  8. Sun, Y., Booker, C.F., Day, R.N. and Periasamy, A. (2009) Localization of protein-protein interactions among three fluorescent proteins in a single living cell: three-color FRET microscopy. SPIE Proc. 7183: 71832E-(1-10).
  9. Wallrabe, H., Periasamy, A. and Barroso, M. (2009) FRET imaging of multiple focal planes to analyze the organization and conformation of tranferrin-receptor in polarized cells. SPIE Proc. 7183: 71832F-(1-6).
  10. Day, R. N., Sun, Y., Booker, C. Y., Kumari, S., Periasamy, A. and Davidson, M. (2009) The characterization of optimized fluorescent proteins for Forster resonance energy transfer microscopy. SPIE Proc. 7183: 718306.
  11. Li, H., Yu, P., Felder, R.A., Periasamy, A. and Jose, P.A. (2009) Angiotension II-induced angiotension II type I receptor lysosomal degradation studied by fluorescence lifetime imaging microscopy. SPIE Proc. 7183: 71832G-(1-12).
  12. Chen, Y. and Periasamy, A. (2007) Acceptor spectral bleedthrough correction in spectral FRET imaging microscopy. SPIE Proc. 6442. 644223-(1-9).
  13. Yen, W., Burdsal, C., Periasamy, A. and Sutherland, A.E. (2006) Axial elongation in mouse embryos involves mediolateral cell intercalation behavior in the paraxial mesoderm. SPIE Proc. 6089:60891K-1-9.
  14. Wallrabe, H., Periasamy, A. and Barroso, M. (2006) 2-P FLIM-FRET in combination with confocal FRET provides additional insights of the organization of Transferrin receptor-ligand complexes in endocytic membranes of polarized MDCK cells. SPIE Proc. 6089:608905-1-9.
  15. Chen, Y. and Periasamy, A. (2006) Quantitative FRET data analysis: Protein localization in living specimens. SPIE Proc. 6089:60891W-1-9.
  16. Chen, Y., King, M., and Periasamy, A. (2005) One- and two-component analysis of cyan fluorescent protein: FLIM-FRET microscopy. SPIE Proc. 5700:279-285.
  17. Chen, Y and Periasamy, A. (2004) Two-photon lifetime imaging microscopy for protein localization. SPIE Proc. 5323, 431-439.
  18. Chen, Y., Majid, S., Day, R.N. and A. Periasamy (2003) Two-photon excitation lifetime imaging and excitation spectra of green fluorescent proteins. SPIE Proc. 4963, 259-260.
  19. Wallrabe, H., Elangovan, M., Burchard, A., Periasamy, A. and Barroso, M. (2002) FRET microscopy reveals clustered distribution of co-internalized receptor-ligand complexes in the apical recyling endosome of polarized epithelial MDCK cells. SPIE Proc. 4620, 64-73.
  20. Elangovan, M., Day, R.N. and Periasamy, A. (2002) FRET-FLIM microscopy. SPIE Proc. 4620, 267-273.
  21. Kneas, K.A., Demas, J.N., DeGraff, B.A. and Periasamy, A. (2001) Comparison of conventional, confocal, and two-photon microscopy for detection of microcrystals within luminescence-based oxygen sensor films (Invited Paper). SPIE Proc. Vol. 4262, 89-97.
  22. Day, R.N. and Periasamy, A. (2001) FRET microscopy to visualize transcription factor dimerization in the nucleus of the living cell (Invited Paper). SPIE Proc. Vol. 4262, 163-170.
  23. Elangovan, M. and Periasamy, A. (2001) Bleed-hrough and photobleaching correction in multiphoton FRET microscopy. SPIE Proc. Vol. 4262, 177-185.
  24. Ganesan, S., Elangovan, M. and Periasamy, A. (2001) Kinetics and comparison of d-aminolevulinic-acid-induced endogenous protoporphyrin-IX in single cell by steady state and multiphoton fluorescence imaging. SPIE Proc. Vol. 4262, 263-267.
  25. Abraham, A.T., Brautigan, D.L., Hecht, S.M. and Periasamy, A. (2001) Localization of bleomycin in a single living cell using three-photon excitation microscopy. SPIE Proc. Vol. 4262, 347-353.
  26. Periasamy, A., Elangovan, M. and Day, R.N. (2001) Comparison of one- and two-photon fluorescence resonance energy transfer microscopy. SPIE Proc. Vol. 4262, 366-372.
  27. Wallrabe, H., Elangovan, M., Periasamy, A. and Barroso, M., (2001) Use of laser scanning confocal and two-photon FRET microscopy to image and quantify the colocalization of fluorophore-labeled ligands in MDCK epithelial cells. SPIE Proc. Vol. 4262, 373-382.
  28. Noakes, C.R., Goto, T., Keller, R. and Periasamy, A. (2001) Does digital deconvolution improve two-photon microscopy in deep tissue imaging? SPIE Proc. Vol. 4262, 389-395.
  29. Periasamy, A. (2000) Two-photon energy transfer microscopy. Proc. SPIE, 3921, 299-304.
  30. Elangovan, M., Peter, K. and Periasamy, A. (2000) Fluorescence lifetime imaging: Simulation and error analysis. Proc. SPIE, 3921, 280-287.
  31. Periasamy, A., Sharman, K.K. and Demas, J.N. (1999) Fluorescence lifetime imaging microscopy using rapid lifetime determination method: Theory and applications. Biophy. J. 76: A10.
  32. Periasamy, A., Sharman, K.K., Ahuja, R. Eto, I. and Brautigan, D.L. (1999) Fluorescence Lifetime Imaging of Green Fluorescent Protein of Living Cells. Proc. SPIE, 3604: 6-12.
  33. Periasamy, A. (1999) Multi-photon excitation fluorescence microscopy in the biomedical sciences. Proc. SPIE, 3604: 74-82.
  34. Periasamy, A. (1998) Digital deconvolution FRET microscopy: 3D visualization of protein-protein interactions in a single living cell. Proc. SPIE, 3260: 106-114.
  35. Periasamy, A., Kay, S.A. and Day, R.N. (1997) Fluorescence resonance energy transfer (FRET) imaging of a single living cell using green fluorescent protein. SPIE Proc., 2983: 58-66.
  36. Periasamy, A., and Day, R. N. (1997) Pit-1 protein localization at different optical sections in a single living cell using FRET microscopy and green fluorescent proteins. In “Proc. Microscopy and Microanalysis” (G. W. Bailey, R. V. W. Dimlich, K. B. Alexander, J. J. McCarthy, T. P. Pretlow, Eds.), Vol. 3 pp. 133-134. Springer-Verlag, New York.
  37. Herman, B., Wang, X.F., Periasamy, A., Kwon, S., Gordon, G.W., Wodnicki, P. (1996) Fluorescence lifetime imaging in cell biology. SPIE Proc., 2678: 88-97.
  38. Wang, X.F., Periasamy, A., Gordon, G.W., Wodnicki, P. and Herman, B. (1994) Fluorescence lifetime imaging microscopy (FLIM) and its applications. SPIE Proc.,      2137: 64-76.
  39. Diliberto, P.A., Periasamy, A. and Herman, A. (1991) Distinct oscillations in cytosolic and nuclear free calcium in single intact living cells demonstrated by confocal microscopy. In: Proc: 49th Ann. Meet. Elect. Micros. Soc. America (EMSA). Ed: G.W.Bailey, San Francisco Press Inc., San Francisco, pp228-229.
  40. Periasamy, A., Lindroth, J. R. and Thompson, R. P. (1988) Real time analysis of video image and electrocardiogram of the chick embryo heart. In: Proc. of the Ann. Int. Conf. IEEE/EMBS, New Orleans, 10: 483-484.
  41. Periasamy, A., Holdren, D. N., Everts, W. C. and Pollack, G. H. (1985) Digital image processing of muscle striations. In:Proc. Annual Int. Conf. IEEE/EMBS, Chicago, 7: 971-975.
  42. Burns, D. H., Holdren, D. N., Periasamy, A., Everts, W. C. and Pollack, G. H. (1985) Image processing of muscle striations below the resolution limit of the microscope. Medical Image Processing/SPIE-593: 46-51.
  43. Ramachandran, G., Periasamy, A. and Singh, M. (1985) Laser speckle displacement cardiography. in:Proc. Annual Int. Conf.IEEE/EMBS, Chicago, 7: 1192-1195.
  44. Burns, D.H., Holdren, D.N., Periasamy, A., Everts, W.C. and Pollack, G.H. (1985) Image processing of muscle striations below the resolution limit of the microscope. Medical image Processing/SPIE 593: 46-51.
  45. Sridharan, K. S., Mothi S. F.., Rajalakshmi, M. G., Natarajan, S. and Periasamy, A. (1984) A learner's aid for studies on bioelectronics. in:Proc. Int. Conf. on Teaching Aids in Phy. Education (TAPE'84), Madras, p24-28.

Abstracts (4)

  1. Rehman, S., Brown-Steinke, K., Periasamy, A. and Palmer, L., (2015) S-Nitrosothiols regulate eNOS trafficking in pulmonary endothelial cells. American Thoracic Society (ATS) International Conference, Denver, Colorado. May 15-20.
  2. Jyothikumar,  V.; Sun, Y.; Periasamy, A. (2014) Analysis of heterogenous NADH fluorescence in live cells during hypoxia. Biophysical Society meeting.
  3. Jyothikumar,  V.; Sun, Y.; Periasamy, A. (2013) Label Free Fluorescence Lifetime FRET Imaging; Biophysical Society meeting, Elsevier., pp671a.
  4. Shagufta Rehman, Kathleen Brown-Steinke, Kimberly deRonde, Steven J. Lewis, Ammasi Periasamy, Scott Randell, Lisa A. Palmer (2015) S-nitrosothiols regulate eNOS trafficking in Pulmonary Endothelial Cells. American Thoracic Society International Conference